Aesthetix Io won't start...

One thought: some of the Io units have fuses mounted on the circuit board inside the case. Later production units eliminated these, but my unit has them. If you're comfortable opening the case, do so and take a look for a typical BUSS style fuse (glass with metal end caps) mounted on the circuit board and check that fuse. Jim now manufactures the Io with this fuse bypassed and inoperable because it tripped to easily and proved not to add any real value in protecting the circuit. I had this fuse trip on me once several years ago and Jim had to tell me about it's existence. I replaced it and have not had another problem.
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Great advice, many thanks Rushton. My power supply has two fuses in the back and two mounted on the board itself, one of them says Buss MDL 3/8. This may be broken, has some black dots on the inside of the glass even if the thread looks whole. I have not found any fuses on the main unit motherboard (this is mk2). Will get new fuses and report back.

Problem solved, thanks to Rushton's advice! It was the Buss fuse that was gone. Great to get help like this.

Great to hear, O_holter!! BTW, Jim says not to worry about anything being wrong if these fuses blow. Just replace them and move on, they are unlikely go out again and it's not an indication of a problem with the unit. This is why he's eliminated them from current production.
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Great to get this kind of help in a forum. Perhaps Aesthetix users could help make a troubleshooting list. for example, how long before reconnecting the power cables, how to search for tube errors, how to replace fuses. Silly me, I didn't catch up to the fact that I was hearing a typical fuse error (no sound) rather than tube error ( bad sound) and used some hours changing tubes. Eventually, I replaced the bad "Buss"-marked fuse with a standard gas station 5 ampere fuse, and have not noticed anything wrong afterwards.