So your sub has L and R inputs. That simplifies it a bit. All you have to do is to run a cable from L out from the Focusrite to L in on the sub. Ditto with R. I am not sure about the plug that you linked to (call Focusrite for advice). See here for more information: https://www.presonus.com/learn/technical-articles/Balanced-Unbalanced
Try the Antimode whenever you feel like it - I think you will love it.
I would cross over rather lower, like 40 Hz for a start, but you can experiment.
The Yamaha AS801 will be a bit easier, but the sound should be virtually the same, with a bit less dynamic headroom in that large room.
Bi-amping is not worth it in your situation, and adds a lot of worries.
An Oppo probably only makes sense if you want to play discs and/or want to connect a TV screen.
Once you have this up and running, stop worrying about the electronics (they will be more than good enough, even with far better speakers). Your three weakest links will be the main speakers (start saving for something like the bigger Harbeths), the room modes generated by the sub (get an Antimode), and the higher frequency reflections because of the hard walls, glass windows etc. (get some curtains, carpets, or whatever).
Try the Antimode whenever you feel like it - I think you will love it.
I would cross over rather lower, like 40 Hz for a start, but you can experiment.
The Yamaha AS801 will be a bit easier, but the sound should be virtually the same, with a bit less dynamic headroom in that large room.
Bi-amping is not worth it in your situation, and adds a lot of worries.
An Oppo probably only makes sense if you want to play discs and/or want to connect a TV screen.
Once you have this up and running, stop worrying about the electronics (they will be more than good enough, even with far better speakers). Your three weakest links will be the main speakers (start saving for something like the bigger Harbeths), the room modes generated by the sub (get an Antimode), and the higher frequency reflections because of the hard walls, glass windows etc. (get some curtains, carpets, or whatever).