Everything else being equal, should I expect better Spotify quality through wifi or USB?

The 2 choices I am looking at are:

1) USB out of desktop computer into DAC
2) Wifi connection to DAC

I am wondering if there would be any difference in streaming Spotify sound quality between these 2 options, using something like a Bluesound Node 2, which can work with either option.

Same DAC? The DAC may have a very small sonic signature (but not much). If the rest is functioning properly, it is highly unlikelely there will be a sonic signature from that part of the chain.

willemj: Did you read my original post?  Yes, same DAC.  My question is written:

If the DAC, amp, speakers are the same, will one of these 2 options likely provide better sound quality?

Sorry I overlooked the 'same DAC'. If I understand you now, this is a DAC with both a usb port and a streaming facility, or, to put it the other way around,  a streamer with its own DAC. I would be surprised if there are any sonic differences, so I would go for the most convenient option.

The short answer is it depends. The variables are the quality and length of your USB cable, the performance of your laptop, WiFi signal strength and internet speed, and the quality of your streamer to a lesser degree. A better comparison is between wired Ethernet connection and WiFi and even that would depend on the quality and length of your Ethernet cable vs. your WiFi signal strength and internet speed. The ultimate arbitrator is your ears.  Just keep in mind to unplug your laptop and run on battery if/when streaming from your computer since the AC noise is a major culprit in introducing noise in the signal chain.