Not when many poweramps use opamps as their balanced inputs, and then it routes to their single ended input.OK George, by this comment I have to assume that opamps are not a thing with which you are familiar. Opamps (operational amplifiers) have very high gain open loop. Their gain structure is thus defined by the feedback resistance vs the input resistance. When both values are the same the opamp has unity gain. This is a lot of feedback, and most opamps today can support that amount of feedback such that at any frequency in the audio band they will be entirely neutral.
In these cases by using the single ended input, you get a better sound, as there’s no opamp in the signal path then.
And unity gain is how such at thing would be set up, since the amplifier in question would have no need of additional gain when using the balanced input. At any rate, if the designer did execute the opamp input in a substandard manner (and I have seen that), such a thing does not reflect on balanced operation nearly so much as it does on that designer!
Take this from someone who made their career in tube electronics, OK?
So the sound quality will be unaffected although I do concede that using an opamp solely for this purpose is a poor execution of the amplifier design; better if the amp simply has differential inputs used for either balanced or single-ended operation (and many in high end do exactly that). McCormick and Pass Labs being two examples off the top of my head...