it may sound unhalanced left to right or not as strong and powerful as it could. if they are over biased the tubes could burn out prematurely.
also it is a good way to spot a bad tube. a bad tube cannot be biased to the correct current.
definitely warm up the amp and at least check the bias of each tube by flipping the individual switches on and off and read the meter. the manual says that they do not have to be perfect so you might not need to adjust the bias but i recommend it