@whart made a couple of points that I agree with. I check the bias frequently. I don’t have to use a fluke meter as my amp has meters for voltage and current for both the driver tubes and the output tubes. My amp is very stable but I have been rolling tubes a lot lately.
I also agree that there is no need to use white gloves. I’ll admit I’m an outdoor guy who doesn’t use lotion so my skin tends to be dry. but most tubes run cool enough that a little skin oil it isn’t a problem.
Thinking about this thread for the last day, I think the biggest factor in tube failure is running them at the wrong voltage or current. Most amps don’t have as much adjustability as mine. I’ve had amps with no adjustability (cathode bias) and they are designed to be easy on tubes--no action required.
I had a Decware ZMA and it had a great biasing system that I really enjoyed. it accommodates a lot of tubes and the bias current for each tube can be different. to calculate the bias current for a particular tube, look up the maximum plate dissipation on the tube data sheet (watts). Measure the B+ voltage of your amp. The best way is to use some insulated probes clipped to the correct pins (tube slightly elevated) running out to a volt meter. Make sure you don’t create a short to the chassis, turn your amp on, write down the voltage, turn your amp off and remove the probes. This number is important to know. Sometimes OEMs will provide it.
Divide your plate dissipation (watts) by B+ voltage (Volts) will give you amps. You’ll have a decimal. Multiply by 1000 to get milliamps. Now decide how hard you want to drive your tubes--usually a number between 60% and 80%- and multiply that times your current and that is where you set the bias. I have found recommended bias numbers well above what I calculated. I have also know guys who ran their bias purposely higher than recommended because they thought it sounded better. I think this is a myth, the "higher must be better" mentality.
If you have auto bias, you’re out of luck. I’ll never buy an amp with autobias.
Jerry