HDM and Mark,
My local materials scientist tells me that certain alchohols will decrease the surface tension of very pure water relative to a PVC surface. This may allow Step 4 to flow more readily deep into groove bottoms and tiny modulations than pure water would do. That might explain the results Tbg and Rushton have heard, which I trust to be real. Neither of these gentlemen has ever posted anything that wasn't honest and helpful for as long as I've known them.
My question was based on the fact that the lower the surface tension of a fluid, the more difficult it becomes to remove a thin film of that fluid from a surface. Any combination of fluid, wetted surface and RCM involves a conflict between the surface tension between fluid and surface and the air velocities produced by the RCM. For any given air velocity, the lower the surface tension, the more fluid will be left behind.
Tbg and Rushton's results and Walker's explanation all make perfect sense. I only wondered (perhaps somewhat academically, as Rushton suggested) about the risks of alchohol traces and the possibility that an alchohol step immediately following the enzymes might be useful. Those questions remain, but it's beyond my knowledge to do more than pose them for anyone who's interested to consider.
Doug
P.S. to Mark, neither Paul nor I had anything to do with designing the AI fluids. They were in the finished form we know today several months before we first saw them. Jim gets 100% credit for the redesign. We're just satisfied users like you or anyone, with no other interest.