My experience is that tube dampers are essential. They can improve low level resolution, microdynamics, and allow higher volumes before distortion limits the volume at which you're comfortable. One thing I've found is that it's important to damp the tubes, but not to add mass that can store vibrational energy and then release it. Specifically, I recommend avoiding those tube damping rings that ARC supplies with their preamp tubes. Substituting Herbies Ultrasonic tube dampers on my ARC REF3 preamp eliminated a smearing quality compared with the ARC o-ring dampers. I mentioned this to ARC when I ordered tube replacements.
I do use very thin o-rings on the brass bases of my Kronzilla T1610 tubes. I find that four of these (1/16" thick and 3-1/2" diameter) made a surprising improvement in the sound, with instruments sounding more like solid and independent sources. Interestingly, when I tried putting those thin o-rings (rated to 450F) on the glass of the tubes, they start to smear the sound. For the glass envelopes, I use three Herbies tube dampers per tube.
There's lots of different types of dampers out there. I'd appreciate the input of those who have compared the Herbies dampers with other designs. One more thing, I've found that damping speaker cables also provides improvements.