George, there is no need to re-apply unless you disconnect. The effect of the treatment does not "fade" over time as long as not disconnected. While the instructions do say you may connect and re-connect multiple times (as John points out), pay attention to what you hear. Sometimes you do need to re-apply upon disconnecting/re-connecting. Lloyd has updated his instructions as follows:
"Before reconnecting, it is beneficial to take a used applicator with the SST wiped off (some will still be embedded in the applicator) and spread over the connector. This will help to distribute the SST that remains on the connector. If you reapply it, make sure there is not excessive SST on the connector. If there is, clean it as directed above before applying more." http://www.walkeraudio.com/sst.htm