Tubes can take up to 100 hours to break in, there is no set time so just wait it out.The rectifier tubes in my Art Audio Diavolo took 200 hours. There where also many Mullard tubes labeled with the Amperex label, so they may be the same tubes. You definetely will hear a difference between a Mullard and a Amperex.If you do not,they might be the same tubes. To answer the question regarding the Audio Research LS-16, yes,the tubes make a large difference.I preferred the Mullards, but that is my taste.
Mike