Tbg, I haven't been able to tell anything about enzyme loss of effectiveness in less than 10 hours. I've never mixed up a batch with the large scoop, only the small. And I often mix a half batch with just half the small scoop to do just a few records. It takes me about 8-10 minutes to do both side of an LP with all four steps (including a double rinse on Step 3) using a VPI RCM for the vacuum between steps. Yes, it does take some time.
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