Maybe I'm just out in left field...
I ALWAYS ask the seller, "What is your best price?" I don't see it as tire kicking, trying to push a seller into negotiating with himself, or whatever. I don't waste a seller's time with a lot of back and forth. I just see that one with an "OBO" is willing to consider a price lower than what he's listed. I don't respond to an ad in the first place unless it's a piece I want to buy (and, will buy if the seller doesn't come across as a total you know what), I have the money, and the seller's Audiogon feedback indicates he's someone I can feel comfortable with.
I simply want to know what the bottom line is, and then I can either buy it, or move on.
In the end, I'm left feeling confused - not that it's rare for me. Put forth an offer which may be construed as a "low ball", which will offend. Or, ask the seller what their best price is, which will also offend. So, which is the lesser of the two evils?