Zender,
There are a number of posts on tube rolling for the Io here on A-Gon and many by Albert Porter who has a TON of experience with this unit. A search on "Aesthetix Io" would likely reveal them. There are a number of different tubes to suit different tastes.
For lower noise, I use, as recommended by Albert and others, Telefunken 12AX7s. You can start in the first gain stage (most important) and then experiment from there. They can also be used in the power supply. Many replace the stock 6SN7s with RCA Red base 5692s (I currently have these but am going to try Brinmars and Ken Rads) and replace the stock 6922s with various 6DJ8/6922/7308s. My friend Kevin Tellecamp at Silent Running Audio and I bought and REALLY like the tubes and great customer service we get from Bruce Winger at BWS Consulting (www.bws-tube.com) who provided all the NOS tubes we needed and guarantees satisfaction.
I am using NOS Amperex EL-34s in the power supply where Albert likes Mullards. I also use a Russian 6922 alternative where Albert like Mullards (I usually like Amperex 7308s but didn't like them in this unit for some reason).
As for the second power supply, it really is a cost/benefit analysis based on your own finances and preferences. FOR me, it added a bit (not a ton) of slam, transparency and slightly improved dynamics. If your funds allow it, go for it. If not, you probably won't be unhappy with one.
As for the 220 conversion, I doubt it would be difficult but I would call Jim White for the real answer.