Hello Shuang,
I haven't noticed one bit of difference, nor did my friend who conducted the software tests, between 2 sprays or 5 sprays of Shine Ola. I don't know why they recommend so many sprays, it is not needed.
Regarding re-application, I never do unless the disc becomes dirty again. Remember, Shine Ola is just a cleaner and does not leave a film that will wear off (or raise BLER). Once cleaned and the mold release agents are removed, it is fine.
I haven't noticed any difference with Shine Ola that would make me believe that it is transport dependant, it works great on everything our group has thrown at it, from a $30 DVD player for the kids to a EMM Labs and various modified SACD players. Some transports will read better, but that defines the ability of the transport, not Shine Ola or any other cleaner.
I've cleaned my 3M cloths with Woolite by hand, (first cleaning my hands with anti-bacterial soap), and a final rinse of the 3M cloth with Record Research Labs Super Vinyl Wash, just because it is the cleanest water that I know of, and therefore contains less contaminates. I then let the 3M cloth air dry using a thumb tack, a string and a safety pin. My Wife is mad about the holes in the ceiling, so I'll have to figure something else out soon, but it works.
Regarding the cyro treatment, I'd re-clean the discs, just because their handling is unknown throughout the entire cryo process. I'd rather be certain that they are clean and sounding as good as they can.
I hope this helps ...