Starting with the speakers in a sub-optimal position, it's easy to discern improvements in the sound. As the speakers approach a more optimal position, the sound improves. It was absolutely something I could hear. Detailed note taking supported the optimum position, as three different times I preferred that location. The 1/3, 1/4, 1/5 room width markers were simply guides, just like the perpendicular strips placed every 6 inches. The final speaker position did not fall exactly on those markers.
And no reference sound was targeted. I am trying to get my system to sound the best it can in the room it is in–my ears being the barometer. 19 different positions is what it took to get one step closer to that goal.
My room is symmetrical, thus why I used a laser measure to precisely place the speakers, and yes, the listening chair too.