I wonder how many people have compared I2S with USB in a well-controlled A/B test. I2S still isn't all that widely available. My streaming DAC does have an I2S input but I've never used it. In my system, the most likely path into that IIS port would be from my Mac's, uh, USB port, via a converter box such as the Sonore ultraDigital or the Matrix X-SPDIF 2. I've been slightly curious to see if that would produce a noticeable improvement, but don't really want to add another little converter box into an already cluttered system. Besides, the SQ already is pretty darn good. But I do wish my music server (the SGC sonicTransporter) had an IIS output port. My DAC with IIS input can support 16-32 bit PCM, while its USB pipeline supports only 16-24 bit. Perhaps more importantly, IIS might eliminate the annoying USB handshake problems I sometimes experience.
IIS from the Matrix X-SPDIF seems to be popular among users of the PS Audio Direct Stream DAC. I wonder if this popularity doesn't stem from limitations of PS Audio's USB implementations, or from problems with the Direct Stream's network bridge card. Point is, again, that if we're going to compare USB to IIS, I think we need to specify the context of how USB is being implemented in the system we're comparing.