Hey guys, really appreciate all your help here. It looks like the first thing I should try is to swop out the Mullard 5AR4; then maybe try some other output tubes, maybe the GEs.
As for my cables, I'm using a Lessloss DFPC power cord for the 5400ES, and a Black Sand Silver Mk V for the power supply. From the power cable forums, it does not seem like these cables would cause my problem. As for ICs, I have tried Pure Note Vanguard and VH Audio fine silver DIY ICs. The latter helps to bring to lift the top end a touch.
In case any of you are interested, the rest of my system is Aesthetix Calypso pre-amp; Krell FPB300c power and Von Schweikert VR4jr speakers. I use a Hydra 8 power conditioner and a separate Hydra 2 for the Krell. I also take a lot of care in component a lot of care taken in system set-up with use of Herbie's accessories equipment & speaker support with accessories from Herbie's Lab.
Thanks again.