External HD Changing the quality of the sound?


Hi everyone! It's my first thread i start in this community and i'm willing to go far in the journey of Hi-Fi audio :)
So i was wondering, is it real that if you put your audio file in an external hd it lower the quality on the outline?
I still don't have dac to test it so i wanted to be sure before putting my library in it, since my external hd is a 2tb and the one i have in my imac is a 500gb and is full...
Also, is it true that the type of connection used for my hd is changing the quality (usb 2.0, usb 3.0, firewire800)?
I know how it could affect it, i just want to know if it is all true?
freb03
Yeap. BitPerfect (click on the little logo on the top right of the menu bar. Select Preferences. You can adjust the size of the cache obviously limited by the RAM installed but RAM is cheap these days. Our old 2011 iMac already had 4x4GB RAM
Doggiehowser - I am just doing some pre-lim exploration of computer audio...and, in another thread, noted your enthusiasm for a set up that sounds somewhat like mine. Hope you can provide additional insight and guidance here about use of Bitperfect.

If I purchase Bitperfect do I need to install it on my external hard drive where my iTunes music library (and iTunes) resides or will it work properly with iTunes (which is operating from the external hard drive) if BitPerfect is simply downloaded to the MacBook Air hard drive? Hope that is not too ignorant a question.

System is: iTunes & Apple Lossless Files on SeaGate 1TB ext. H/D>>MB Air>> USB out>>M/F V-Link>>M/F DAC>>Pre-amp.

Thanks in advance for your help.
BitPerfect is an app that works alongside iTunes. You use iTunes to manage your library (set which HDD to keep the library in etc, add new songs, add metadata info if needed, create playlists etc).

BitPerfect just takes over the playback engine from iTunes. You install it from the App Store like with most new apps. It will be in the Applications folder.