“But is there a big difference of sound quality if we have optical isolation between the shared PC and an endpoint ?”
This would squarely depends on your rest of the system. You may hear the difference by upgrading to NUC or you may not. I doubt you would get any consensus to your dilemma. For me, both NUC or PC is a non-starter. A dedicated streamer like Innuos or Lumin along with a decent DAC is a must if you’re serious about getting the best out of cloud based streaming services like Qobuz or Tidal.
Once you accomplish above, then you can tweak your way into optimizing the Ethernet signal before it reaches your streamer.