My list doesn't exactly follow the Op's list (my apologies, but then, I never have followed the rules) but the improvement I experienced when each was implemented was significant enough to warrant a mention.
Most important tweak in my opinion, though probably not an actual tweak is dealing with room acoustics. I dealt with mine electronically instead of acoustically because of cost, floor space and WAF. I do plan to explore acoustic options in the future.
The next biggest improvement was when I switched to fully balanced operation. The improvement in tone, dynamics and system noise was completely unexpected.
This doesn't fall into the tweak category either but the improvement was significant enough that I feel it needs to be mentioned, adding an active crossover between subs and mains. The difference in clarity and tightness in the low end was rather eye opening
Time aligning the subs and mains added even more clarity, space between instruments and improved imaging.
My last tweak is actually on the Op's list, component isolation. I haven't done extensive isolation and because of my penchant for loud, bass driven music the isolation platform under my TT is more of a necessity than a tweak. Other components I have used minimal isolation on so far are my subs and amps. I also have plans to explore isolation under my other components and improve the isolation under the subs and amps.